pbabe puro braf v600e cat 15269 plasmids (Addgene inc)
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pbabe puro braf v600e cat 15269 plasmids
Pbabe Puro Braf V600e Cat 15269 Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbabe+puro+braf+v600e/pBabe-Puro-BRAF-V600E+(Plasmid+%2315269)/pm39374781-141-18-25
Average 93 stars, based on 44 article reviews
Pbabe Puro Braf V600e Cat 15269 Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbabe+puro+braf+v600e/pBabe-Puro-BRAF-V600E+(Plasmid+%2315269)/pm39374781-141-18-25
Average 93 stars, based on 44 article reviews
pbabe puro braf v600e cat 15269 plasmids - by Bioz Stars,
2026-10
93/100 stars
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Polymerase Chain Reaction:Article Title: Reporter cell lines to screen for inhibitors or regulators of the KRAS-RAF-MEK1/2-ERK1/2 pathway Article Snippet: For inducible expression of oncogenes we used the the Flp-In TRex system from Invitrogen (ThermoFisher Scientific Cat No K650001). .. To generate HeLa cells with doxycycline-inducible BRAFV600E, BRAF1799T>A (encoding BRAFV600E) was PCR amplfii ed from Article Title: Reporter cell lines to screen for inhibitors or regulators of the KRAS-RAF-MEK1/2-ERK1/2 pathway Article Snippet: .. To generate HeLa cells with doxycycline-inducible BRAF V600E , BRAF 1799T>A (encoding BRAF V600E ) was PCR amplfii ed from Plasmid Preparation:Article Title: Reporter cell lines to screen for inhibitors or regulators of the KRAS-RAF-MEK1/2-ERK1/2 pathway Article Snippet: For inducible expression of oncogenes we used the the Flp-In TRex system from Invitrogen (ThermoFisher Scientific Cat No K650001). .. To generate HeLa cells with doxycycline-inducible BRAFV600E, BRAF1799T>A (encoding BRAFV600E) was PCR amplfii ed from Article Title: Clonal dynamics of BRAF -driven drug resistance in EGFR -mutant lung cancer Article Snippet: Twenty-four hour after infection, the growth medium was changed and 3 μg/ml (PC9) or 2 μg/ml (HCC827) puromycin (Sigma Aldrich, USA, Cat. No. p8833) was added for selection for 7 days. .. After selection, cells were analyzed for protein expression. pBABE-puro was a gift from Hartmut Land & Jay Morgenstern & Bob Weinberg (Addgene plasmid # 1764 ; RRID:Addgene_1764). Article Title: Reporter cell lines to screen for inhibitors or regulators of the KRAS-RAF-MEK1/2-ERK1/2 pathway Article Snippet: .. To generate HeLa cells with doxycycline-inducible BRAF V600E , BRAF 1799T>A (encoding BRAF V600E ) was PCR amplfii ed from Clone Assay:Article Title: Reporter cell lines to screen for inhibitors or regulators of the KRAS-RAF-MEK1/2-ERK1/2 pathway Article Snippet: For inducible expression of oncogenes we used the the Flp-In TRex system from Invitrogen (ThermoFisher Scientific Cat No K650001). .. To generate HeLa cells with doxycycline-inducible BRAFV600E, BRAF1799T>A (encoding BRAFV600E) was PCR amplfii ed from Article Title: USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K. Article Snippet: .. BEAS-2B oncogenic cells were generated upon retroviral infection of BEAS-2B differentiated (DIF) with the next plasmids: EGFR (addgene number: #11011), EGFR L858R (addgene number: #11012), pBabe puro HA PIK3CA (addgene number: #12522), pBabe puro HA PIK3CA H1047R (addgene number: #12524), pBabe puro HA PIK3CA E545K (addgene number: #12525), pBabe puro HRAS G12D (HRAS G12D was cloned into pBabe puro in our laboratory) and Article Title: USP28 enables oncogenic transformation of respiratory cells and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K Article Snippet: .. BEAS-2B Oncogenic cells were generated upon retroviral infection of BEAS-2B DIF with the next plasmids: EGFR (addgene number: #11011), EGFR L858R (addgene number: #11012), pBabe puro HA PIK3CA (addgene number: #12522), pBabe puro HA PIK3CA H1047R (addgene number: #12524), pBabe puro HA PIK3CA E545K (addgene number: #12525), pBabe puro HRAS G12D (HRAS G12D was cloned into pBabe puro in our lab) and Article Title: Reporter cell lines to screen for inhibitors or regulators of the KRAS-RAF-MEK1/2-ERK1/2 pathway Article Snippet: .. To generate HeLa cells with doxycycline-inducible BRAF V600E , BRAF 1799T>A (encoding BRAF V600E ) was PCR amplfii ed from Article Title: USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K Article Snippet: .. BEAS‐2B oncogenic cells were generated upon retroviral infection of BEAS‐2B differentiated (DIF) with the next plasmids: EGFR (addgene number: #11011), EGFR L858R (addgene number: #11012), pBabe puro HA PIK3CA (addgene number: #12522), pBabe puro HA PIK3CA H1047R (addgene number: #12524), pBabe puro HA PIK3CA E545K (addgene number: #12525), pBabe puro HRAS G12D (HRAS G12D was cloned into pBabe puro in our laboratory) and Sequencing:Article Title: Reporter cell lines to screen for inhibitors or regulators of the KRAS-RAF-MEK1/2-ERK1/2 pathway Article Snippet: For inducible expression of oncogenes we used the the Flp-In TRex system from Invitrogen (ThermoFisher Scientific Cat No K650001). .. To generate HeLa cells with doxycycline-inducible BRAFV600E, BRAF1799T>A (encoding BRAFV600E) was PCR amplfii ed from Article Title: Reporter cell lines to screen for inhibitors or regulators of the KRAS-RAF-MEK1/2-ERK1/2 pathway Article Snippet: .. To generate HeLa cells with doxycycline-inducible BRAF V600E , BRAF 1799T>A (encoding BRAF V600E ) was PCR amplfii ed from Generated:Article Title: USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K. Article Snippet: .. BEAS-2B oncogenic cells were generated upon retroviral infection of BEAS-2B differentiated (DIF) with the next plasmids: EGFR (addgene number: #11011), EGFR L858R (addgene number: #11012), pBabe puro HA PIK3CA (addgene number: #12522), pBabe puro HA PIK3CA H1047R (addgene number: #12524), pBabe puro HA PIK3CA E545K (addgene number: #12525), pBabe puro HRAS G12D (HRAS G12D was cloned into pBabe puro in our laboratory) and Article Title: USP28 enables oncogenic transformation of respiratory cells and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K Article Snippet: .. BEAS-2B Oncogenic cells were generated upon retroviral infection of BEAS-2B DIF with the next plasmids: EGFR (addgene number: #11011), EGFR L858R (addgene number: #11012), pBabe puro HA PIK3CA (addgene number: #12522), pBabe puro HA PIK3CA H1047R (addgene number: #12524), pBabe puro HA PIK3CA E545K (addgene number: #12525), pBabe puro HRAS G12D (HRAS G12D was cloned into pBabe puro in our lab) and Article Title: USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K Article Snippet: .. BEAS‐2B oncogenic cells were generated upon retroviral infection of BEAS‐2B differentiated (DIF) with the next plasmids: EGFR (addgene number: #11011), EGFR L858R (addgene number: #11012), pBabe puro HA PIK3CA (addgene number: #12522), pBabe puro HA PIK3CA H1047R (addgene number: #12524), pBabe puro HA PIK3CA E545K (addgene number: #12525), pBabe puro HRAS G12D (HRAS G12D was cloned into pBabe puro in our laboratory) and Retroviral:Article Title: USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K. Article Snippet: .. BEAS-2B oncogenic cells were generated upon retroviral infection of BEAS-2B differentiated (DIF) with the next plasmids: EGFR (addgene number: #11011), EGFR L858R (addgene number: #11012), pBabe puro HA PIK3CA (addgene number: #12522), pBabe puro HA PIK3CA H1047R (addgene number: #12524), pBabe puro HA PIK3CA E545K (addgene number: #12525), pBabe puro HRAS G12D (HRAS G12D was cloned into pBabe puro in our laboratory) and Article Title: USP28 enables oncogenic transformation of respiratory cells and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K Article Snippet: .. BEAS-2B Oncogenic cells were generated upon retroviral infection of BEAS-2B DIF with the next plasmids: EGFR (addgene number: #11011), EGFR L858R (addgene number: #11012), pBabe puro HA PIK3CA (addgene number: #12522), pBabe puro HA PIK3CA H1047R (addgene number: #12524), pBabe puro HA PIK3CA E545K (addgene number: #12525), pBabe puro HRAS G12D (HRAS G12D was cloned into pBabe puro in our lab) and Article Title: USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K Article Snippet: .. BEAS‐2B oncogenic cells were generated upon retroviral infection of BEAS‐2B differentiated (DIF) with the next plasmids: EGFR (addgene number: #11011), EGFR L858R (addgene number: #11012), pBabe puro HA PIK3CA (addgene number: #12522), pBabe puro HA PIK3CA H1047R (addgene number: #12524), pBabe puro HA PIK3CA E545K (addgene number: #12525), pBabe puro HRAS G12D (HRAS G12D was cloned into pBabe puro in our laboratory) and Infection:Article Title: USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K. Article Snippet: .. BEAS-2B oncogenic cells were generated upon retroviral infection of BEAS-2B differentiated (DIF) with the next plasmids: EGFR (addgene number: #11011), EGFR L858R (addgene number: #11012), pBabe puro HA PIK3CA (addgene number: #12522), pBabe puro HA PIK3CA H1047R (addgene number: #12524), pBabe puro HA PIK3CA E545K (addgene number: #12525), pBabe puro HRAS G12D (HRAS G12D was cloned into pBabe puro in our laboratory) and Article Title: USP28 enables oncogenic transformation of respiratory cells and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K Article Snippet: .. BEAS-2B Oncogenic cells were generated upon retroviral infection of BEAS-2B DIF with the next plasmids: EGFR (addgene number: #11011), EGFR L858R (addgene number: #11012), pBabe puro HA PIK3CA (addgene number: #12522), pBabe puro HA PIK3CA H1047R (addgene number: #12524), pBabe puro HA PIK3CA E545K (addgene number: #12525), pBabe puro HRAS G12D (HRAS G12D was cloned into pBabe puro in our lab) and Article Title: USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K Article Snippet: .. BEAS‐2B oncogenic cells were generated upon retroviral infection of BEAS‐2B differentiated (DIF) with the next plasmids: EGFR (addgene number: #11011), EGFR L858R (addgene number: #11012), pBabe puro HA PIK3CA (addgene number: #12522), pBabe puro HA PIK3CA H1047R (addgene number: #12524), pBabe puro HA PIK3CA E545K (addgene number: #12525), pBabe puro HRAS G12D (HRAS G12D was cloned into pBabe puro in our laboratory) and Cell Culture:Article Title: Characterisation of cellular effects of Burkholderia pseudomallei cycle inhibiting factor (Cif) Article Snippet: .. Cells were cultured in 12-well plates and transfected with 0.8 μg empty vector pcDNA3.1, V5-Cif-pcDNA3.1, V5-Cif C156S-pcDNA3.1, dnUBC12-HA C111S-pcDNA3 , pBabe-Puro-MEK-DD or Transfection:Article Title: Characterisation of cellular effects of Burkholderia pseudomallei cycle inhibiting factor (Cif) Article Snippet: .. Cells were cultured in 12-well plates and transfected with 0.8 μg empty vector pcDNA3.1, V5-Cif-pcDNA3.1, V5-Cif C156S-pcDNA3.1, dnUBC12-HA C111S-pcDNA3 , pBabe-Puro-MEK-DD or Selection:Article Title: Clonal dynamics of BRAF -driven drug resistance in EGFR -mutant lung cancer Article Snippet: Twenty-four hour after infection, the growth medium was changed and 3 μg/ml (PC9) or 2 μg/ml (HCC827) puromycin (Sigma Aldrich, USA, Cat. No. p8833) was added for selection for 7 days. .. After selection, cells were analyzed for protein expression. pBABE-puro was a gift from Hartmut Land & Jay Morgenstern & Bob Weinberg (Addgene plasmid # 1764 ; RRID:Addgene_1764). Expressing:Article Title: Clonal dynamics of BRAF -driven drug resistance in EGFR -mutant lung cancer Article Snippet: Twenty-four hour after infection, the growth medium was changed and 3 μg/ml (PC9) or 2 μg/ml (HCC827) puromycin (Sigma Aldrich, USA, Cat. No. p8833) was added for selection for 7 days. .. After selection, cells were analyzed for protein expression. pBABE-puro was a gift from Hartmut Land & Jay Morgenstern & Bob Weinberg (Addgene plasmid # 1764 ; RRID:Addgene_1764). |